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Saturday, 23 May 2020

Detecting Red Blood Cell Damage in Real-time


According to the National Kidney Foundation, more than 37 million people are living with kidney disease.

The kidneys play an important role in the body, from removing waste products to filtering the blood. For people with kidney disease, dialysis can help the body perform these essential functions when the kidneys aren't working at full capacity.

However, red blood cells sometimes rupture when blood is sent through faulty equipment that is supposed to clean the blood, such as a dialysis machine. This is called hemolysis. Hemolysis also can occur during blood work when blood is drawn too quickly through a needle, leading to defective laboratory samples.

There is no reliable indicator that red blood cells are being damaged in a clinical setting until an individual begins showing symptoms, such as fever, weakness, dizziness or confusion.



University of Delaware mechanical engineer Tyler Van Buren and collaborating colleagues at Princeton University have developed a method to monitor blood damage in real-time.

"Our goal was to find a method that could detect red blood cell damage without the need for lab sample testing," said Van Buren, an assistant professor of mechanical engineering with expertise in fluid dynamics.

Detecting blood cell damage

In the body, red blood cells float in plasma alongside white blood cells and platelets. The plasma is naturally conductive and is efficient at passing an electric charge. Red blood cells are chock-full of hemoglobin, an oxygen-transporting protein, that also is conductive.

This hemoglobin is typically insulated from the body by the cell lining. But as red blood cells rupture, hemoglobin is released into the bloodstream, causing the blood to become more conductive.

"Think of the blood like a river and red blood cells like water balloons in that river," said Van Buren, who joined UD in 2019. "If you have electrons (negatively charged particles) waiting to cross the river, it is more difficult when there are a lot of water balloons present. This is because the rubber is insulated, so the blood will be less conductive. As the water balloons (or blood cells) break, there are fewer barriers and the blood becomes more conductive, making it easier for electrons to move from one side to the other."

This diagram depicts the way conductivity will change as blood cells break. The yellow dots represent electrons. The red circles represent blood cells. Viewing the graphic from left to right, one can see that when more blood cells are present, fewer electrons are able to get across. As blood cells break, there are fewer barriers and the blood becomes more conductive, making it easier for electrons to move from one side to the other.


In dialysis, a patient's blood is removed from the body, cleaned, then recirculated into the body. The researchers developed a simple experiment to see if they could measure the blood's mechanical resistance outside of the body.

To test their technique, the researchers circulated healthy blood through the laboratory system and gradually introduced mechanically damaged blood to see if it would change the conductive nature of the fluid in the system.

It did. The researchers saw a direct correlation between the conductivity of the fluid in the system and the amount of damaged blood included in the sample.

While this issue of damaged blood is very rare, the research team's method does introduce one potential way to indirectly monitor blood damage in the body during dialysis. The researchers theorize that if clinicians were able to monitor the resistance of a patient's blood going into a dialysis machine and coming out, and they saw a major change in resistance -- or conductivity -- there is good reason to believe the blood is being damaged.

"We are not doctors, we're mechanical engineers," said Van Buren. "This technique would need a lot more vetting before being applied in a clinical setting."

For example, Van Buren said the method wouldn't necessarily work across patient populations because an individual's blood conductivity is just that, individual.



In the future, Van Buren said it would be interesting to evaluate whether conductivity also could be used in place of lab sampling for applications outside of dialysis. For example, this might be useful in research aimed at understanding how blood cells may be damaged, both inside and outside of the body, and possible methods for prevention.

He also is curious whether this method could be used to evaluate and identify compromised blood samples on-site, saving time and money for hospitals or diagnostic laboratories, while eliminating the need for patients to make multiple trips to have blood drawn if there is a problem.


Bibliography:

A simple method to monitor hemolysis in real time

Tyler Van Buren, Gilad Arwatz & Alexander J. Smits

Scientific Reports volume 10, Article number: 5101

doi: 10.1038/s41598-020-62041-8

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